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| Name |
Modeling 3D Distortion of a Fluorescence Microscope and CCD Imaging: Theory to Practice |
| Comments |
Fluorescence microscopy imaging is widely used in medical research for studying micro-scale biological organisms. In practice, images captured by fluorescence microscopes become aberrated or distorted at the CCD camera output.
Microscope out-of-focus light contributes a significant amount of distortion characterizing the so-called point-spread-function (PSF), which is the three-dimensional (3D) impulse response of the fluorescence microscope system. In addition, in practice what we observe is the result of the whole system comprising the microscope and the CCD camera. In existing algorithms of microscopy-deconvolution, researchers discard contributions from the CCD camera. Modeling 3D PSF of the whole system will have a significant impact on deconvolution-microscopy.
In this project, we will investigate a new scheme for measuring and computing the distortion function/matrix of the combined system of fluorescence microscope and CCD camera. We will perform imaging of fluorescent beads, signal processing, and computation based analysis.
Students are required to have basic knowledge in signal processing, linear algebra, some statistics, and MATLAB programming. |
| WebPage |
not specified
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| Application |
not specified
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| Appl Deadline |
date not specified
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| Keywords |
Fluorescence microscopy micro-scale biological organisms aberrated distorted CCD point-spread-function |
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| College |
School of Engineering & Applied Science |
| Department |
Electrical and Systems Engineering
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| Professor |
Nehorai, Arye
[ email ]
[ web page ]
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| Start Time |
not specified
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| End Time |
not specified
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| Hours Per Week |
0 hour(s)
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| Number Positions |
not specified
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| Credits |
3 hour(s)
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| Prerequisites |
No Prerequisites
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| Internship |
Not an Internship
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| Stipend |
No stipend available
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